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mouse monoclonal anti mcherry  (TaKaRa)


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    Structured Review

    TaKaRa mouse monoclonal anti mcherry
    Reagents and tools table
    Mouse Monoclonal Anti Mcherry, supplied by TaKaRa, used in various techniques. Bioz Stars score: 97/100, based on 257 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/mouse+anti+mcherry+monoclonal+antibody/Living+Colors+mCherry+Monoclonal+Antibody/pmc13672799-41-0-4
    Average 97 stars, based on 257 article reviews
    mouse monoclonal anti mcherry - by Bioz Stars, 2026-10
    97/100 stars

    Images

    1) Product Images from "Excess Met1-linked ubiquitination leads to solid aggregate formation"

    Article Title: Excess Met1-linked ubiquitination leads to solid aggregate formation

    Journal: The EMBO Journal

    doi: 10.1038/s44318-026-00909-7

    Reagents and tools table
    Figure Legend Snippet: Reagents and tools table

    Techniques Used:

    Related Articles

    Immunofluorescence:

    Article Title: A single-cell transcriptomic atlas reveals resident dendritic-like cells in the zebrafish brain parenchyma
    Article Snippet: Immunostaining was performed on 14 μm cryosections as previously described ( ).Immunostaining was performed on 14 μm cryosections as previously described ( ).. The following primary and secondary antibodies were used: chicken anti-GFP polyclonal antibody (1:500; Abcam, Cat# ab13970), rabbit anti-Lcp1 (1:1000), mouse anti-mCherry monoclonal antibody (1:500; Takara Bio Cat# 632543), Alexa Fluor 488-conjugated anti-chicken IgG antibody (1:500; Abcam Cat# ab150169), Alexa Fluor 594-conjugated anti-rabbit IgG (1:500; Abcam Cat# ab150076), Alexa Fluor 647-conjugated anti-mouse IgG (1:500; Abcam Cat# ab150107).. For vibratome sections, adult brains were fixed in 4% PFA, embedded in 7% low-melting agarose in PBS, and sectioned at 100 μm using a vibratome (Leica).For vibratome sections, adult brains were fixed in 4% PFA, embedded in 7% low-melting agarose in PBS, and sectioned at 100 μm using a vibratome (Leica).

    Article Title: Learning-dependent structural plasticity of intracortical and sensory connections to functional domains of the olfactory tubercle
    Article Snippet: with 0.2% Triton-X (TBST) containing 10% normal goat serum, and incubated with primary antibodies in blocking buffer overnight at room temperature. .. The primary antibodies were mouse anti-Cre recombinase monoclonal antibody (1:200, MAB3120; Merck Millipore, Darmstadt, Germany), rabbit anti-c-fos polyclonal antibody (1:500; ab-190,289; Abcam, Cambridge, United Kingdom), mouse anti-mCherry monoclonal antibody (1:500; #632543; Takara Bio, Shiga, Japan), guinea pig anti-VGLUT1 polyclonal antibody (1:500; AB5905; Merck Millipore, Burlington, MA, United States), and rabbit anti-Homer 1b/c antibody (1:200; #160023; Synaptic Systems, Goettingen, Germany). .. The sections were washed with TBST and then incubated with appropriate fluorescent dye (Alexa)-conjugated secondary antibodies (1:300; Invitrogen, Wal

    Article Title: A single-cell transcriptomic atlas reveals resident dendritic-like cells in the zebrafish brain parenchyma
    Article Snippet: was performed on 14 µm cryosections as described ( ). .. The following primary and secondary antibodies were used: chicken anti-GFP polyclonal antibody (1:500; Abcam, Cat# ab13970), rabbit anti-Lcp1 (1:1000), mouse anti-mCherry monoclonal antibody (1:500; Takara Bio Cat# 632543), Alexa Fluor 488-conjugated anti-chicken IgG antibody (1:500; Abcam Cat# ab150169), Alexa Fluor 594-conjugated anti-rabbit IgG (1:500; Abcam Cat# ab150076), Alexa Fluor 647-conjugated anti-mouse IgG (1:500; Abcam Cat# ab150107). .. For vibratome sections, adult brains were fixed in 4% PFA and include

    Article Title: Learning-dependent structural plasticity of intracortical and sensory connections to functional domains of the olfactory tubercle.
    Article Snippet: For immunohistochemical analysis, dried sections were rehydrated in PBS, blocked with Tris-buffered saline with 0.2% Triton-X (TBST) containing 10% normal goat serum, and incubated with primary antibodies in blocking buffer overnight at room temperature.dried sections were rehydrated in PBS, blocked with Tris-buffered saline with 0.2% Triton-X (TBST) containing 10% normal goat serum, and incubated with primary antibodies in blocking buffer overnight at room temperature. ... The primary antibodies were mouse anti-Cre recombinase monoclonal antibody (1:200, MAB3120; Merck Millipore, Darmstadt, Germany), rabbit anti-c-fos polyclonal antibody (1:500; ab-190,289; Abcam, Cambridge, United Kingdom), mouse anti-mCherry monoclonal antibody (1:500; #632543; Takara Bio, Shiga, Japan), guinea pig antiVGLUT1 polyclonal antibody (1:500; AB5905; Merck Millipore, Burlington, MA, United States), and rabbit anti-Homer 1b/c antibody (1:200; #160023; Synaptic Systems, Goettingen, Germany).. The sections were washed with TBST and then incubated with appropriate fluorescent dye (Alexa)-conjugated secondary antibodies (1:300; Invitrogen, Waltham, MA, United States) for 1 h at room temperature.The sections were washed with TBST and then incubated with appropriate fluorescent dye (Alexa)-conjugated secondary antibodies (1:300; Invitrogen, Waltham, MA, United States) for 1 h at room temperature.

    Transgenic Assay:

    Article Title: A single-cell transcriptomic atlas reveals resident dendritic-like cells in the zebrafish brain parenchyma
    Article Snippet: Immunostaining was performed on 14 μm cryosections as previously described ( ).Immunostaining was performed on 14 μm cryosections as previously described ( ).. The following primary and secondary antibodies were used: chicken anti-GFP polyclonal antibody (1:500; Abcam, Cat# ab13970), rabbit anti-Lcp1 (1:1000), mouse anti-mCherry monoclonal antibody (1:500; Takara Bio Cat# 632543), Alexa Fluor 488-conjugated anti-chicken IgG antibody (1:500; Abcam Cat# ab150169), Alexa Fluor 594-conjugated anti-rabbit IgG (1:500; Abcam Cat# ab150076), Alexa Fluor 647-conjugated anti-mouse IgG (1:500; Abcam Cat# ab150107).. For vibratome sections, adult brains were fixed in 4% PFA, embedded in 7% low-melting agarose in PBS, and sectioned at 100 μm using a vibratome (Leica).For vibratome sections, adult brains were fixed in 4% PFA, embedded in 7% low-melting agarose in PBS, and sectioned at 100 μm using a vibratome (Leica).

    Article Title: Learning-dependent structural plasticity of intracortical and sensory connections to functional domains of the olfactory tubercle
    Article Snippet: with 0.2% Triton-X (TBST) containing 10% normal goat serum, and incubated with primary antibodies in blocking buffer overnight at room temperature. .. The primary antibodies were mouse anti-Cre recombinase monoclonal antibody (1:200, MAB3120; Merck Millipore, Darmstadt, Germany), rabbit anti-c-fos polyclonal antibody (1:500; ab-190,289; Abcam, Cambridge, United Kingdom), mouse anti-mCherry monoclonal antibody (1:500; #632543; Takara Bio, Shiga, Japan), guinea pig anti-VGLUT1 polyclonal antibody (1:500; AB5905; Merck Millipore, Burlington, MA, United States), and rabbit anti-Homer 1b/c antibody (1:200; #160023; Synaptic Systems, Goettingen, Germany). .. The sections were washed with TBST and then incubated with appropriate fluorescent dye (Alexa)-conjugated secondary antibodies (1:300; Invitrogen, Wal

    Article Title: A single-cell transcriptomic atlas reveals resident dendritic-like cells in the zebrafish brain parenchyma
    Article Snippet: was performed on 14 µm cryosections as described ( ). .. The following primary and secondary antibodies were used: chicken anti-GFP polyclonal antibody (1:500; Abcam, Cat# ab13970), rabbit anti-Lcp1 (1:1000), mouse anti-mCherry monoclonal antibody (1:500; Takara Bio Cat# 632543), Alexa Fluor 488-conjugated anti-chicken IgG antibody (1:500; Abcam Cat# ab150169), Alexa Fluor 594-conjugated anti-rabbit IgG (1:500; Abcam Cat# ab150076), Alexa Fluor 647-conjugated anti-mouse IgG (1:500; Abcam Cat# ab150107). .. For vibratome sections, adult brains were fixed in 4% PFA and include

    Article Title: Learning-dependent structural plasticity of intracortical and sensory connections to functional domains of the olfactory tubercle.
    Article Snippet: For immunohistochemical analysis, dried sections were rehydrated in PBS, blocked with Tris-buffered saline with 0.2% Triton-X (TBST) containing 10% normal goat serum, and incubated with primary antibodies in blocking buffer overnight at room temperature.dried sections were rehydrated in PBS, blocked with Tris-buffered saline with 0.2% Triton-X (TBST) containing 10% normal goat serum, and incubated with primary antibodies in blocking buffer overnight at room temperature. ... The primary antibodies were mouse anti-Cre recombinase monoclonal antibody (1:200, MAB3120; Merck Millipore, Darmstadt, Germany), rabbit anti-c-fos polyclonal antibody (1:500; ab-190,289; Abcam, Cambridge, United Kingdom), mouse anti-mCherry monoclonal antibody (1:500; #632543; Takara Bio, Shiga, Japan), guinea pig antiVGLUT1 polyclonal antibody (1:500; AB5905; Merck Millipore, Burlington, MA, United States), and rabbit anti-Homer 1b/c antibody (1:200; #160023; Synaptic Systems, Goettingen, Germany).. The sections were washed with TBST and then incubated with appropriate fluorescent dye (Alexa)-conjugated secondary antibodies (1:300; Invitrogen, Waltham, MA, United States) for 1 h at room temperature.The sections were washed with TBST and then incubated with appropriate fluorescent dye (Alexa)-conjugated secondary antibodies (1:300; Invitrogen, Waltham, MA, United States) for 1 h at room temperature.

    Immunostaining:

    Article Title: A single-cell transcriptomic atlas reveals resident dendritic-like cells in the zebrafish brain parenchyma
    Article Snippet: Immunostaining was performed on 14 μm cryosections as previously described ( ).Immunostaining was performed on 14 μm cryosections as previously described ( ).. The following primary and secondary antibodies were used: chicken anti-GFP polyclonal antibody (1:500; Abcam, Cat# ab13970), rabbit anti-Lcp1 (1:1000), mouse anti-mCherry monoclonal antibody (1:500; Takara Bio Cat# 632543), Alexa Fluor 488-conjugated anti-chicken IgG antibody (1:500; Abcam Cat# ab150169), Alexa Fluor 594-conjugated anti-rabbit IgG (1:500; Abcam Cat# ab150076), Alexa Fluor 647-conjugated anti-mouse IgG (1:500; Abcam Cat# ab150107).. For vibratome sections, adult brains were fixed in 4% PFA, embedded in 7% low-melting agarose in PBS, and sectioned at 100 μm using a vibratome (Leica).For vibratome sections, adult brains were fixed in 4% PFA, embedded in 7% low-melting agarose in PBS, and sectioned at 100 μm using a vibratome (Leica).

    Article Title: Learning-dependent structural plasticity of intracortical and sensory connections to functional domains of the olfactory tubercle
    Article Snippet: with 0.2% Triton-X (TBST) containing 10% normal goat serum, and incubated with primary antibodies in blocking buffer overnight at room temperature. .. The primary antibodies were mouse anti-Cre recombinase monoclonal antibody (1:200, MAB3120; Merck Millipore, Darmstadt, Germany), rabbit anti-c-fos polyclonal antibody (1:500; ab-190,289; Abcam, Cambridge, United Kingdom), mouse anti-mCherry monoclonal antibody (1:500; #632543; Takara Bio, Shiga, Japan), guinea pig anti-VGLUT1 polyclonal antibody (1:500; AB5905; Merck Millipore, Burlington, MA, United States), and rabbit anti-Homer 1b/c antibody (1:200; #160023; Synaptic Systems, Goettingen, Germany). .. The sections were washed with TBST and then incubated with appropriate fluorescent dye (Alexa)-conjugated secondary antibodies (1:300; Invitrogen, Wal

    Article Title: A single-cell transcriptomic atlas reveals resident dendritic-like cells in the zebrafish brain parenchyma
    Article Snippet: was performed on 14 µm cryosections as described ( ). .. The following primary and secondary antibodies were used: chicken anti-GFP polyclonal antibody (1:500; Abcam, Cat# ab13970), rabbit anti-Lcp1 (1:1000), mouse anti-mCherry monoclonal antibody (1:500; Takara Bio Cat# 632543), Alexa Fluor 488-conjugated anti-chicken IgG antibody (1:500; Abcam Cat# ab150169), Alexa Fluor 594-conjugated anti-rabbit IgG (1:500; Abcam Cat# ab150076), Alexa Fluor 647-conjugated anti-mouse IgG (1:500; Abcam Cat# ab150107). .. For vibratome sections, adult brains were fixed in 4% PFA and include

    Article Title: Learning-dependent structural plasticity of intracortical and sensory connections to functional domains of the olfactory tubercle.
    Article Snippet: For immunohistochemical analysis, dried sections were rehydrated in PBS, blocked with Tris-buffered saline with 0.2% Triton-X (TBST) containing 10% normal goat serum, and incubated with primary antibodies in blocking buffer overnight at room temperature.dried sections were rehydrated in PBS, blocked with Tris-buffered saline with 0.2% Triton-X (TBST) containing 10% normal goat serum, and incubated with primary antibodies in blocking buffer overnight at room temperature. ... The primary antibodies were mouse anti-Cre recombinase monoclonal antibody (1:200, MAB3120; Merck Millipore, Darmstadt, Germany), rabbit anti-c-fos polyclonal antibody (1:500; ab-190,289; Abcam, Cambridge, United Kingdom), mouse anti-mCherry monoclonal antibody (1:500; #632543; Takara Bio, Shiga, Japan), guinea pig antiVGLUT1 polyclonal antibody (1:500; AB5905; Merck Millipore, Burlington, MA, United States), and rabbit anti-Homer 1b/c antibody (1:200; #160023; Synaptic Systems, Goettingen, Germany).. The sections were washed with TBST and then incubated with appropriate fluorescent dye (Alexa)-conjugated secondary antibodies (1:300; Invitrogen, Waltham, MA, United States) for 1 h at room temperature.The sections were washed with TBST and then incubated with appropriate fluorescent dye (Alexa)-conjugated secondary antibodies (1:300; Invitrogen, Waltham, MA, United States) for 1 h at room temperature.

    Expressing:

    Article Title: A single-cell transcriptomic atlas reveals resident dendritic-like cells in the zebrafish brain parenchyma
    Article Snippet: Immunostaining was performed on 14 μm cryosections as previously described ( ).Immunostaining was performed on 14 μm cryosections as previously described ( ).. The following primary and secondary antibodies were used: chicken anti-GFP polyclonal antibody (1:500; Abcam, Cat# ab13970), rabbit anti-Lcp1 (1:1000), mouse anti-mCherry monoclonal antibody (1:500; Takara Bio Cat# 632543), Alexa Fluor 488-conjugated anti-chicken IgG antibody (1:500; Abcam Cat# ab150169), Alexa Fluor 594-conjugated anti-rabbit IgG (1:500; Abcam Cat# ab150076), Alexa Fluor 647-conjugated anti-mouse IgG (1:500; Abcam Cat# ab150107).. For vibratome sections, adult brains were fixed in 4% PFA, embedded in 7% low-melting agarose in PBS, and sectioned at 100 μm using a vibratome (Leica).For vibratome sections, adult brains were fixed in 4% PFA, embedded in 7% low-melting agarose in PBS, and sectioned at 100 μm using a vibratome (Leica).

    Article Title: Learning-dependent structural plasticity of intracortical and sensory connections to functional domains of the olfactory tubercle
    Article Snippet: with 0.2% Triton-X (TBST) containing 10% normal goat serum, and incubated with primary antibodies in blocking buffer overnight at room temperature. .. The primary antibodies were mouse anti-Cre recombinase monoclonal antibody (1:200, MAB3120; Merck Millipore, Darmstadt, Germany), rabbit anti-c-fos polyclonal antibody (1:500; ab-190,289; Abcam, Cambridge, United Kingdom), mouse anti-mCherry monoclonal antibody (1:500; #632543; Takara Bio, Shiga, Japan), guinea pig anti-VGLUT1 polyclonal antibody (1:500; AB5905; Merck Millipore, Burlington, MA, United States), and rabbit anti-Homer 1b/c antibody (1:200; #160023; Synaptic Systems, Goettingen, Germany). .. The sections were washed with TBST and then incubated with appropriate fluorescent dye (Alexa)-conjugated secondary antibodies (1:300; Invitrogen, Wal

    Article Title: A single-cell transcriptomic atlas reveals resident dendritic-like cells in the zebrafish brain parenchyma
    Article Snippet: was performed on 14 µm cryosections as described ( ). .. The following primary and secondary antibodies were used: chicken anti-GFP polyclonal antibody (1:500; Abcam, Cat# ab13970), rabbit anti-Lcp1 (1:1000), mouse anti-mCherry monoclonal antibody (1:500; Takara Bio Cat# 632543), Alexa Fluor 488-conjugated anti-chicken IgG antibody (1:500; Abcam Cat# ab150169), Alexa Fluor 594-conjugated anti-rabbit IgG (1:500; Abcam Cat# ab150076), Alexa Fluor 647-conjugated anti-mouse IgG (1:500; Abcam Cat# ab150107). .. For vibratome sections, adult brains were fixed in 4% PFA and include

    Article Title: Learning-dependent structural plasticity of intracortical and sensory connections to functional domains of the olfactory tubercle.
    Article Snippet: For immunohistochemical analysis, dried sections were rehydrated in PBS, blocked with Tris-buffered saline with 0.2% Triton-X (TBST) containing 10% normal goat serum, and incubated with primary antibodies in blocking buffer overnight at room temperature.dried sections were rehydrated in PBS, blocked with Tris-buffered saline with 0.2% Triton-X (TBST) containing 10% normal goat serum, and incubated with primary antibodies in blocking buffer overnight at room temperature. ... The primary antibodies were mouse anti-Cre recombinase monoclonal antibody (1:200, MAB3120; Merck Millipore, Darmstadt, Germany), rabbit anti-c-fos polyclonal antibody (1:500; ab-190,289; Abcam, Cambridge, United Kingdom), mouse anti-mCherry monoclonal antibody (1:500; #632543; Takara Bio, Shiga, Japan), guinea pig antiVGLUT1 polyclonal antibody (1:500; AB5905; Merck Millipore, Burlington, MA, United States), and rabbit anti-Homer 1b/c antibody (1:200; #160023; Synaptic Systems, Goettingen, Germany).. The sections were washed with TBST and then incubated with appropriate fluorescent dye (Alexa)-conjugated secondary antibodies (1:300; Invitrogen, Waltham, MA, United States) for 1 h at room temperature.The sections were washed with TBST and then incubated with appropriate fluorescent dye (Alexa)-conjugated secondary antibodies (1:300; Invitrogen, Waltham, MA, United States) for 1 h at room temperature.

    Labeling:

    Article Title: A single-cell transcriptomic atlas reveals resident dendritic-like cells in the zebrafish brain parenchyma
    Article Snippet: Immunostaining was performed on 14 μm cryosections as previously described ( ).Immunostaining was performed on 14 μm cryosections as previously described ( ).. The following primary and secondary antibodies were used: chicken anti-GFP polyclonal antibody (1:500; Abcam, Cat# ab13970), rabbit anti-Lcp1 (1:1000), mouse anti-mCherry monoclonal antibody (1:500; Takara Bio Cat# 632543), Alexa Fluor 488-conjugated anti-chicken IgG antibody (1:500; Abcam Cat# ab150169), Alexa Fluor 594-conjugated anti-rabbit IgG (1:500; Abcam Cat# ab150076), Alexa Fluor 647-conjugated anti-mouse IgG (1:500; Abcam Cat# ab150107).. For vibratome sections, adult brains were fixed in 4% PFA, embedded in 7% low-melting agarose in PBS, and sectioned at 100 μm using a vibratome (Leica).For vibratome sections, adult brains were fixed in 4% PFA, embedded in 7% low-melting agarose in PBS, and sectioned at 100 μm using a vibratome (Leica).

    Article Title: Learning-dependent structural plasticity of intracortical and sensory connections to functional domains of the olfactory tubercle
    Article Snippet: with 0.2% Triton-X (TBST) containing 10% normal goat serum, and incubated with primary antibodies in blocking buffer overnight at room temperature. .. The primary antibodies were mouse anti-Cre recombinase monoclonal antibody (1:200, MAB3120; Merck Millipore, Darmstadt, Germany), rabbit anti-c-fos polyclonal antibody (1:500; ab-190,289; Abcam, Cambridge, United Kingdom), mouse anti-mCherry monoclonal antibody (1:500; #632543; Takara Bio, Shiga, Japan), guinea pig anti-VGLUT1 polyclonal antibody (1:500; AB5905; Merck Millipore, Burlington, MA, United States), and rabbit anti-Homer 1b/c antibody (1:200; #160023; Synaptic Systems, Goettingen, Germany). .. The sections were washed with TBST and then incubated with appropriate fluorescent dye (Alexa)-conjugated secondary antibodies (1:300; Invitrogen, Wal

    Article Title: A single-cell transcriptomic atlas reveals resident dendritic-like cells in the zebrafish brain parenchyma
    Article Snippet: was performed on 14 µm cryosections as described ( ). .. The following primary and secondary antibodies were used: chicken anti-GFP polyclonal antibody (1:500; Abcam, Cat# ab13970), rabbit anti-Lcp1 (1:1000), mouse anti-mCherry monoclonal antibody (1:500; Takara Bio Cat# 632543), Alexa Fluor 488-conjugated anti-chicken IgG antibody (1:500; Abcam Cat# ab150169), Alexa Fluor 594-conjugated anti-rabbit IgG (1:500; Abcam Cat# ab150076), Alexa Fluor 647-conjugated anti-mouse IgG (1:500; Abcam Cat# ab150107). .. For vibratome sections, adult brains were fixed in 4% PFA and include

    Article Title: Learning-dependent structural plasticity of intracortical and sensory connections to functional domains of the olfactory tubercle.
    Article Snippet: For immunohistochemical analysis, dried sections were rehydrated in PBS, blocked with Tris-buffered saline with 0.2% Triton-X (TBST) containing 10% normal goat serum, and incubated with primary antibodies in blocking buffer overnight at room temperature.dried sections were rehydrated in PBS, blocked with Tris-buffered saline with 0.2% Triton-X (TBST) containing 10% normal goat serum, and incubated with primary antibodies in blocking buffer overnight at room temperature. ... The primary antibodies were mouse anti-Cre recombinase monoclonal antibody (1:200, MAB3120; Merck Millipore, Darmstadt, Germany), rabbit anti-c-fos polyclonal antibody (1:500; ab-190,289; Abcam, Cambridge, United Kingdom), mouse anti-mCherry monoclonal antibody (1:500; #632543; Takara Bio, Shiga, Japan), guinea pig antiVGLUT1 polyclonal antibody (1:500; AB5905; Merck Millipore, Burlington, MA, United States), and rabbit anti-Homer 1b/c antibody (1:200; #160023; Synaptic Systems, Goettingen, Germany).. The sections were washed with TBST and then incubated with appropriate fluorescent dye (Alexa)-conjugated secondary antibodies (1:300; Invitrogen, Waltham, MA, United States) for 1 h at room temperature.The sections were washed with TBST and then incubated with appropriate fluorescent dye (Alexa)-conjugated secondary antibodies (1:300; Invitrogen, Waltham, MA, United States) for 1 h at room temperature.

    Imaging:

    Article Title: A single-cell transcriptomic atlas reveals resident dendritic-like cells in the zebrafish brain parenchyma
    Article Snippet: Immunostaining was performed on 14 μm cryosections as previously described ( ).Immunostaining was performed on 14 μm cryosections as previously described ( ).. The following primary and secondary antibodies were used: chicken anti-GFP polyclonal antibody (1:500; Abcam, Cat# ab13970), rabbit anti-Lcp1 (1:1000), mouse anti-mCherry monoclonal antibody (1:500; Takara Bio Cat# 632543), Alexa Fluor 488-conjugated anti-chicken IgG antibody (1:500; Abcam Cat# ab150169), Alexa Fluor 594-conjugated anti-rabbit IgG (1:500; Abcam Cat# ab150076), Alexa Fluor 647-conjugated anti-mouse IgG (1:500; Abcam Cat# ab150107).. For vibratome sections, adult brains were fixed in 4% PFA, embedded in 7% low-melting agarose in PBS, and sectioned at 100 μm using a vibratome (Leica).For vibratome sections, adult brains were fixed in 4% PFA, embedded in 7% low-melting agarose in PBS, and sectioned at 100 μm using a vibratome (Leica).

    Article Title: Learning-dependent structural plasticity of intracortical and sensory connections to functional domains of the olfactory tubercle
    Article Snippet: with 0.2% Triton-X (TBST) containing 10% normal goat serum, and incubated with primary antibodies in blocking buffer overnight at room temperature. .. The primary antibodies were mouse anti-Cre recombinase monoclonal antibody (1:200, MAB3120; Merck Millipore, Darmstadt, Germany), rabbit anti-c-fos polyclonal antibody (1:500; ab-190,289; Abcam, Cambridge, United Kingdom), mouse anti-mCherry monoclonal antibody (1:500; #632543; Takara Bio, Shiga, Japan), guinea pig anti-VGLUT1 polyclonal antibody (1:500; AB5905; Merck Millipore, Burlington, MA, United States), and rabbit anti-Homer 1b/c antibody (1:200; #160023; Synaptic Systems, Goettingen, Germany). .. The sections were washed with TBST and then incubated with appropriate fluorescent dye (Alexa)-conjugated secondary antibodies (1:300; Invitrogen, Wal

    Article Title: A single-cell transcriptomic atlas reveals resident dendritic-like cells in the zebrafish brain parenchyma
    Article Snippet: was performed on 14 µm cryosections as described ( ). .. The following primary and secondary antibodies were used: chicken anti-GFP polyclonal antibody (1:500; Abcam, Cat# ab13970), rabbit anti-Lcp1 (1:1000), mouse anti-mCherry monoclonal antibody (1:500; Takara Bio Cat# 632543), Alexa Fluor 488-conjugated anti-chicken IgG antibody (1:500; Abcam Cat# ab150169), Alexa Fluor 594-conjugated anti-rabbit IgG (1:500; Abcam Cat# ab150076), Alexa Fluor 647-conjugated anti-mouse IgG (1:500; Abcam Cat# ab150107). .. For vibratome sections, adult brains were fixed in 4% PFA and include

    Article Title: Learning-dependent structural plasticity of intracortical and sensory connections to functional domains of the olfactory tubercle.
    Article Snippet: For immunohistochemical analysis, dried sections were rehydrated in PBS, blocked with Tris-buffered saline with 0.2% Triton-X (TBST) containing 10% normal goat serum, and incubated with primary antibodies in blocking buffer overnight at room temperature.dried sections were rehydrated in PBS, blocked with Tris-buffered saline with 0.2% Triton-X (TBST) containing 10% normal goat serum, and incubated with primary antibodies in blocking buffer overnight at room temperature. ... The primary antibodies were mouse anti-Cre recombinase monoclonal antibody (1:200, MAB3120; Merck Millipore, Darmstadt, Germany), rabbit anti-c-fos polyclonal antibody (1:500; ab-190,289; Abcam, Cambridge, United Kingdom), mouse anti-mCherry monoclonal antibody (1:500; #632543; Takara Bio, Shiga, Japan), guinea pig antiVGLUT1 polyclonal antibody (1:500; AB5905; Merck Millipore, Burlington, MA, United States), and rabbit anti-Homer 1b/c antibody (1:200; #160023; Synaptic Systems, Goettingen, Germany).. The sections were washed with TBST and then incubated with appropriate fluorescent dye (Alexa)-conjugated secondary antibodies (1:300; Invitrogen, Waltham, MA, United States) for 1 h at room temperature.The sections were washed with TBST and then incubated with appropriate fluorescent dye (Alexa)-conjugated secondary antibodies (1:300; Invitrogen, Waltham, MA, United States) for 1 h at room temperature.

    Mutagenesis:

    Article Title: A single-cell transcriptomic atlas reveals resident dendritic-like cells in the zebrafish brain parenchyma
    Article Snippet: Immunostaining was performed on 14 μm cryosections as previously described ( ).Immunostaining was performed on 14 μm cryosections as previously described ( ).. The following primary and secondary antibodies were used: chicken anti-GFP polyclonal antibody (1:500; Abcam, Cat# ab13970), rabbit anti-Lcp1 (1:1000), mouse anti-mCherry monoclonal antibody (1:500; Takara Bio Cat# 632543), Alexa Fluor 488-conjugated anti-chicken IgG antibody (1:500; Abcam Cat# ab150169), Alexa Fluor 594-conjugated anti-rabbit IgG (1:500; Abcam Cat# ab150076), Alexa Fluor 647-conjugated anti-mouse IgG (1:500; Abcam Cat# ab150107).. For vibratome sections, adult brains were fixed in 4% PFA, embedded in 7% low-melting agarose in PBS, and sectioned at 100 μm using a vibratome (Leica).For vibratome sections, adult brains were fixed in 4% PFA, embedded in 7% low-melting agarose in PBS, and sectioned at 100 μm using a vibratome (Leica).

    Article Title: Learning-dependent structural plasticity of intracortical and sensory connections to functional domains of the olfactory tubercle
    Article Snippet: with 0.2% Triton-X (TBST) containing 10% normal goat serum, and incubated with primary antibodies in blocking buffer overnight at room temperature. .. The primary antibodies were mouse anti-Cre recombinase monoclonal antibody (1:200, MAB3120; Merck Millipore, Darmstadt, Germany), rabbit anti-c-fos polyclonal antibody (1:500; ab-190,289; Abcam, Cambridge, United Kingdom), mouse anti-mCherry monoclonal antibody (1:500; #632543; Takara Bio, Shiga, Japan), guinea pig anti-VGLUT1 polyclonal antibody (1:500; AB5905; Merck Millipore, Burlington, MA, United States), and rabbit anti-Homer 1b/c antibody (1:200; #160023; Synaptic Systems, Goettingen, Germany). .. The sections were washed with TBST and then incubated with appropriate fluorescent dye (Alexa)-conjugated secondary antibodies (1:300; Invitrogen, Wal

    Article Title: A single-cell transcriptomic atlas reveals resident dendritic-like cells in the zebrafish brain parenchyma
    Article Snippet: was performed on 14 µm cryosections as described ( ). .. The following primary and secondary antibodies were used: chicken anti-GFP polyclonal antibody (1:500; Abcam, Cat# ab13970), rabbit anti-Lcp1 (1:1000), mouse anti-mCherry monoclonal antibody (1:500; Takara Bio Cat# 632543), Alexa Fluor 488-conjugated anti-chicken IgG antibody (1:500; Abcam Cat# ab150169), Alexa Fluor 594-conjugated anti-rabbit IgG (1:500; Abcam Cat# ab150076), Alexa Fluor 647-conjugated anti-mouse IgG (1:500; Abcam Cat# ab150107). .. For vibratome sections, adult brains were fixed in 4% PFA and include

    Article Title: Learning-dependent structural plasticity of intracortical and sensory connections to functional domains of the olfactory tubercle.
    Article Snippet: For immunohistochemical analysis, dried sections were rehydrated in PBS, blocked with Tris-buffered saline with 0.2% Triton-X (TBST) containing 10% normal goat serum, and incubated with primary antibodies in blocking buffer overnight at room temperature.dried sections were rehydrated in PBS, blocked with Tris-buffered saline with 0.2% Triton-X (TBST) containing 10% normal goat serum, and incubated with primary antibodies in blocking buffer overnight at room temperature. ... The primary antibodies were mouse anti-Cre recombinase monoclonal antibody (1:200, MAB3120; Merck Millipore, Darmstadt, Germany), rabbit anti-c-fos polyclonal antibody (1:500; ab-190,289; Abcam, Cambridge, United Kingdom), mouse anti-mCherry monoclonal antibody (1:500; #632543; Takara Bio, Shiga, Japan), guinea pig antiVGLUT1 polyclonal antibody (1:500; AB5905; Merck Millipore, Burlington, MA, United States), and rabbit anti-Homer 1b/c antibody (1:200; #160023; Synaptic Systems, Goettingen, Germany).. The sections were washed with TBST and then incubated with appropriate fluorescent dye (Alexa)-conjugated secondary antibodies (1:300; Invitrogen, Waltham, MA, United States) for 1 h at room temperature.The sections were washed with TBST and then incubated with appropriate fluorescent dye (Alexa)-conjugated secondary antibodies (1:300; Invitrogen, Waltham, MA, United States) for 1 h at room temperature.

    Control:

    Article Title: A single-cell transcriptomic atlas reveals resident dendritic-like cells in the zebrafish brain parenchyma
    Article Snippet: Immunostaining was performed on 14 μm cryosections as previously described ( ).Immunostaining was performed on 14 μm cryosections as previously described ( ).. The following primary and secondary antibodies were used: chicken anti-GFP polyclonal antibody (1:500; Abcam, Cat# ab13970), rabbit anti-Lcp1 (1:1000), mouse anti-mCherry monoclonal antibody (1:500; Takara Bio Cat# 632543), Alexa Fluor 488-conjugated anti-chicken IgG antibody (1:500; Abcam Cat# ab150169), Alexa Fluor 594-conjugated anti-rabbit IgG (1:500; Abcam Cat# ab150076), Alexa Fluor 647-conjugated anti-mouse IgG (1:500; Abcam Cat# ab150107).. For vibratome sections, adult brains were fixed in 4% PFA, embedded in 7% low-melting agarose in PBS, and sectioned at 100 μm using a vibratome (Leica).For vibratome sections, adult brains were fixed in 4% PFA, embedded in 7% low-melting agarose in PBS, and sectioned at 100 μm using a vibratome (Leica).

    Article Title: Learning-dependent structural plasticity of intracortical and sensory connections to functional domains of the olfactory tubercle
    Article Snippet: with 0.2% Triton-X (TBST) containing 10% normal goat serum, and incubated with primary antibodies in blocking buffer overnight at room temperature. .. The primary antibodies were mouse anti-Cre recombinase monoclonal antibody (1:200, MAB3120; Merck Millipore, Darmstadt, Germany), rabbit anti-c-fos polyclonal antibody (1:500; ab-190,289; Abcam, Cambridge, United Kingdom), mouse anti-mCherry monoclonal antibody (1:500; #632543; Takara Bio, Shiga, Japan), guinea pig anti-VGLUT1 polyclonal antibody (1:500; AB5905; Merck Millipore, Burlington, MA, United States), and rabbit anti-Homer 1b/c antibody (1:200; #160023; Synaptic Systems, Goettingen, Germany). .. The sections were washed with TBST and then incubated with appropriate fluorescent dye (Alexa)-conjugated secondary antibodies (1:300; Invitrogen, Wal

    Article Title: A single-cell transcriptomic atlas reveals resident dendritic-like cells in the zebrafish brain parenchyma
    Article Snippet: was performed on 14 µm cryosections as described ( ). .. The following primary and secondary antibodies were used: chicken anti-GFP polyclonal antibody (1:500; Abcam, Cat# ab13970), rabbit anti-Lcp1 (1:1000), mouse anti-mCherry monoclonal antibody (1:500; Takara Bio Cat# 632543), Alexa Fluor 488-conjugated anti-chicken IgG antibody (1:500; Abcam Cat# ab150169), Alexa Fluor 594-conjugated anti-rabbit IgG (1:500; Abcam Cat# ab150076), Alexa Fluor 647-conjugated anti-mouse IgG (1:500; Abcam Cat# ab150107). .. For vibratome sections, adult brains were fixed in 4% PFA and include

    Article Title: Learning-dependent structural plasticity of intracortical and sensory connections to functional domains of the olfactory tubercle.
    Article Snippet: For immunohistochemical analysis, dried sections were rehydrated in PBS, blocked with Tris-buffered saline with 0.2% Triton-X (TBST) containing 10% normal goat serum, and incubated with primary antibodies in blocking buffer overnight at room temperature.dried sections were rehydrated in PBS, blocked with Tris-buffered saline with 0.2% Triton-X (TBST) containing 10% normal goat serum, and incubated with primary antibodies in blocking buffer overnight at room temperature. ... The primary antibodies were mouse anti-Cre recombinase monoclonal antibody (1:200, MAB3120; Merck Millipore, Darmstadt, Germany), rabbit anti-c-fos polyclonal antibody (1:500; ab-190,289; Abcam, Cambridge, United Kingdom), mouse anti-mCherry monoclonal antibody (1:500; #632543; Takara Bio, Shiga, Japan), guinea pig antiVGLUT1 polyclonal antibody (1:500; AB5905; Merck Millipore, Burlington, MA, United States), and rabbit anti-Homer 1b/c antibody (1:200; #160023; Synaptic Systems, Goettingen, Germany).. The sections were washed with TBST and then incubated with appropriate fluorescent dye (Alexa)-conjugated secondary antibodies (1:300; Invitrogen, Waltham, MA, United States) for 1 h at room temperature.The sections were washed with TBST and then incubated with appropriate fluorescent dye (Alexa)-conjugated secondary antibodies (1:300; Invitrogen, Waltham, MA, United States) for 1 h at room temperature.

    MANN-WHITNEY:

    Article Title: A single-cell transcriptomic atlas reveals resident dendritic-like cells in the zebrafish brain parenchyma
    Article Snippet: Immunostaining was performed on 14 μm cryosections as previously described ( ).Immunostaining was performed on 14 μm cryosections as previously described ( ).. The following primary and secondary antibodies were used: chicken anti-GFP polyclonal antibody (1:500; Abcam, Cat# ab13970), rabbit anti-Lcp1 (1:1000), mouse anti-mCherry monoclonal antibody (1:500; Takara Bio Cat# 632543), Alexa Fluor 488-conjugated anti-chicken IgG antibody (1:500; Abcam Cat# ab150169), Alexa Fluor 594-conjugated anti-rabbit IgG (1:500; Abcam Cat# ab150076), Alexa Fluor 647-conjugated anti-mouse IgG (1:500; Abcam Cat# ab150107).. For vibratome sections, adult brains were fixed in 4% PFA, embedded in 7% low-melting agarose in PBS, and sectioned at 100 μm using a vibratome (Leica).For vibratome sections, adult brains were fixed in 4% PFA, embedded in 7% low-melting agarose in PBS, and sectioned at 100 μm using a vibratome (Leica).

    Article Title: Learning-dependent structural plasticity of intracortical and sensory connections to functional domains of the olfactory tubercle
    Article Snippet: with 0.2% Triton-X (TBST) containing 10% normal goat serum, and incubated with primary antibodies in blocking buffer overnight at room temperature. .. The primary antibodies were mouse anti-Cre recombinase monoclonal antibody (1:200, MAB3120; Merck Millipore, Darmstadt, Germany), rabbit anti-c-fos polyclonal antibody (1:500; ab-190,289; Abcam, Cambridge, United Kingdom), mouse anti-mCherry monoclonal antibody (1:500; #632543; Takara Bio, Shiga, Japan), guinea pig anti-VGLUT1 polyclonal antibody (1:500; AB5905; Merck Millipore, Burlington, MA, United States), and rabbit anti-Homer 1b/c antibody (1:200; #160023; Synaptic Systems, Goettingen, Germany). .. The sections were washed with TBST and then incubated with appropriate fluorescent dye (Alexa)-conjugated secondary antibodies (1:300; Invitrogen, Wal

    Article Title: A single-cell transcriptomic atlas reveals resident dendritic-like cells in the zebrafish brain parenchyma
    Article Snippet: was performed on 14 µm cryosections as described ( ). .. The following primary and secondary antibodies were used: chicken anti-GFP polyclonal antibody (1:500; Abcam, Cat# ab13970), rabbit anti-Lcp1 (1:1000), mouse anti-mCherry monoclonal antibody (1:500; Takara Bio Cat# 632543), Alexa Fluor 488-conjugated anti-chicken IgG antibody (1:500; Abcam Cat# ab150169), Alexa Fluor 594-conjugated anti-rabbit IgG (1:500; Abcam Cat# ab150076), Alexa Fluor 647-conjugated anti-mouse IgG (1:500; Abcam Cat# ab150107). .. For vibratome sections, adult brains were fixed in 4% PFA and include

    Article Title: Learning-dependent structural plasticity of intracortical and sensory connections to functional domains of the olfactory tubercle.
    Article Snippet: For immunohistochemical analysis, dried sections were rehydrated in PBS, blocked with Tris-buffered saline with 0.2% Triton-X (TBST) containing 10% normal goat serum, and incubated with primary antibodies in blocking buffer overnight at room temperature.dried sections were rehydrated in PBS, blocked with Tris-buffered saline with 0.2% Triton-X (TBST) containing 10% normal goat serum, and incubated with primary antibodies in blocking buffer overnight at room temperature. ... The primary antibodies were mouse anti-Cre recombinase monoclonal antibody (1:200, MAB3120; Merck Millipore, Darmstadt, Germany), rabbit anti-c-fos polyclonal antibody (1:500; ab-190,289; Abcam, Cambridge, United Kingdom), mouse anti-mCherry monoclonal antibody (1:500; #632543; Takara Bio, Shiga, Japan), guinea pig antiVGLUT1 polyclonal antibody (1:500; AB5905; Merck Millipore, Burlington, MA, United States), and rabbit anti-Homer 1b/c antibody (1:200; #160023; Synaptic Systems, Goettingen, Germany).. The sections were washed with TBST and then incubated with appropriate fluorescent dye (Alexa)-conjugated secondary antibodies (1:300; Invitrogen, Waltham, MA, United States) for 1 h at room temperature.The sections were washed with TBST and then incubated with appropriate fluorescent dye (Alexa)-conjugated secondary antibodies (1:300; Invitrogen, Waltham, MA, United States) for 1 h at room temperature.

    Two Tailed Test:

    Article Title: A single-cell transcriptomic atlas reveals resident dendritic-like cells in the zebrafish brain parenchyma
    Article Snippet: Immunostaining was performed on 14 μm cryosections as previously described ( ).Immunostaining was performed on 14 μm cryosections as previously described ( ).. The following primary and secondary antibodies were used: chicken anti-GFP polyclonal antibody (1:500; Abcam, Cat# ab13970), rabbit anti-Lcp1 (1:1000), mouse anti-mCherry monoclonal antibody (1:500; Takara Bio Cat# 632543), Alexa Fluor 488-conjugated anti-chicken IgG antibody (1:500; Abcam Cat# ab150169), Alexa Fluor 594-conjugated anti-rabbit IgG (1:500; Abcam Cat# ab150076), Alexa Fluor 647-conjugated anti-mouse IgG (1:500; Abcam Cat# ab150107).. For vibratome sections, adult brains were fixed in 4% PFA, embedded in 7% low-melting agarose in PBS, and sectioned at 100 μm using a vibratome (Leica).For vibratome sections, adult brains were fixed in 4% PFA, embedded in 7% low-melting agarose in PBS, and sectioned at 100 μm using a vibratome (Leica).

    Article Title: Learning-dependent structural plasticity of intracortical and sensory connections to functional domains of the olfactory tubercle
    Article Snippet: with 0.2% Triton-X (TBST) containing 10% normal goat serum, and incubated with primary antibodies in blocking buffer overnight at room temperature. .. The primary antibodies were mouse anti-Cre recombinase monoclonal antibody (1:200, MAB3120; Merck Millipore, Darmstadt, Germany), rabbit anti-c-fos polyclonal antibody (1:500; ab-190,289; Abcam, Cambridge, United Kingdom), mouse anti-mCherry monoclonal antibody (1:500; #632543; Takara Bio, Shiga, Japan), guinea pig anti-VGLUT1 polyclonal antibody (1:500; AB5905; Merck Millipore, Burlington, MA, United States), and rabbit anti-Homer 1b/c antibody (1:200; #160023; Synaptic Systems, Goettingen, Germany). .. The sections were washed with TBST and then incubated with appropriate fluorescent dye (Alexa)-conjugated secondary antibodies (1:300; Invitrogen, Wal

    Article Title: A single-cell transcriptomic atlas reveals resident dendritic-like cells in the zebrafish brain parenchyma
    Article Snippet: was performed on 14 µm cryosections as described ( ). .. The following primary and secondary antibodies were used: chicken anti-GFP polyclonal antibody (1:500; Abcam, Cat# ab13970), rabbit anti-Lcp1 (1:1000), mouse anti-mCherry monoclonal antibody (1:500; Takara Bio Cat# 632543), Alexa Fluor 488-conjugated anti-chicken IgG antibody (1:500; Abcam Cat# ab150169), Alexa Fluor 594-conjugated anti-rabbit IgG (1:500; Abcam Cat# ab150076), Alexa Fluor 647-conjugated anti-mouse IgG (1:500; Abcam Cat# ab150107). .. For vibratome sections, adult brains were fixed in 4% PFA and include

    Article Title: Learning-dependent structural plasticity of intracortical and sensory connections to functional domains of the olfactory tubercle.
    Article Snippet: For immunohistochemical analysis, dried sections were rehydrated in PBS, blocked with Tris-buffered saline with 0.2% Triton-X (TBST) containing 10% normal goat serum, and incubated with primary antibodies in blocking buffer overnight at room temperature.dried sections were rehydrated in PBS, blocked with Tris-buffered saline with 0.2% Triton-X (TBST) containing 10% normal goat serum, and incubated with primary antibodies in blocking buffer overnight at room temperature. ... The primary antibodies were mouse anti-Cre recombinase monoclonal antibody (1:200, MAB3120; Merck Millipore, Darmstadt, Germany), rabbit anti-c-fos polyclonal antibody (1:500; ab-190,289; Abcam, Cambridge, United Kingdom), mouse anti-mCherry monoclonal antibody (1:500; #632543; Takara Bio, Shiga, Japan), guinea pig antiVGLUT1 polyclonal antibody (1:500; AB5905; Merck Millipore, Burlington, MA, United States), and rabbit anti-Homer 1b/c antibody (1:200; #160023; Synaptic Systems, Goettingen, Germany).. The sections were washed with TBST and then incubated with appropriate fluorescent dye (Alexa)-conjugated secondary antibodies (1:300; Invitrogen, Waltham, MA, United States) for 1 h at room temperature.The sections were washed with TBST and then incubated with appropriate fluorescent dye (Alexa)-conjugated secondary antibodies (1:300; Invitrogen, Waltham, MA, United States) for 1 h at room temperature.

    Flow Cytometry:

    Article Title: A single-cell transcriptomic atlas reveals resident dendritic-like cells in the zebrafish brain parenchyma
    Article Snippet: Immunostaining was performed on 14 μm cryosections as previously described ( ).Immunostaining was performed on 14 μm cryosections as previously described ( ).. The following primary and secondary antibodies were used: chicken anti-GFP polyclonal antibody (1:500; Abcam, Cat# ab13970), rabbit anti-Lcp1 (1:1000), mouse anti-mCherry monoclonal antibody (1:500; Takara Bio Cat# 632543), Alexa Fluor 488-conjugated anti-chicken IgG antibody (1:500; Abcam Cat# ab150169), Alexa Fluor 594-conjugated anti-rabbit IgG (1:500; Abcam Cat# ab150076), Alexa Fluor 647-conjugated anti-mouse IgG (1:500; Abcam Cat# ab150107).. For vibratome sections, adult brains were fixed in 4% PFA, embedded in 7% low-melting agarose in PBS, and sectioned at 100 μm using a vibratome (Leica).For vibratome sections, adult brains were fixed in 4% PFA, embedded in 7% low-melting agarose in PBS, and sectioned at 100 μm using a vibratome (Leica).

    Article Title: Learning-dependent structural plasticity of intracortical and sensory connections to functional domains of the olfactory tubercle
    Article Snippet: with 0.2% Triton-X (TBST) containing 10% normal goat serum, and incubated with primary antibodies in blocking buffer overnight at room temperature. .. The primary antibodies were mouse anti-Cre recombinase monoclonal antibody (1:200, MAB3120; Merck Millipore, Darmstadt, Germany), rabbit anti-c-fos polyclonal antibody (1:500; ab-190,289; Abcam, Cambridge, United Kingdom), mouse anti-mCherry monoclonal antibody (1:500; #632543; Takara Bio, Shiga, Japan), guinea pig anti-VGLUT1 polyclonal antibody (1:500; AB5905; Merck Millipore, Burlington, MA, United States), and rabbit anti-Homer 1b/c antibody (1:200; #160023; Synaptic Systems, Goettingen, Germany). .. The sections were washed with TBST and then incubated with appropriate fluorescent dye (Alexa)-conjugated secondary antibodies (1:300; Invitrogen, Wal

    Article Title: A single-cell transcriptomic atlas reveals resident dendritic-like cells in the zebrafish brain parenchyma
    Article Snippet: was performed on 14 µm cryosections as described ( ). .. The following primary and secondary antibodies were used: chicken anti-GFP polyclonal antibody (1:500; Abcam, Cat# ab13970), rabbit anti-Lcp1 (1:1000), mouse anti-mCherry monoclonal antibody (1:500; Takara Bio Cat# 632543), Alexa Fluor 488-conjugated anti-chicken IgG antibody (1:500; Abcam Cat# ab150169), Alexa Fluor 594-conjugated anti-rabbit IgG (1:500; Abcam Cat# ab150076), Alexa Fluor 647-conjugated anti-mouse IgG (1:500; Abcam Cat# ab150107). .. For vibratome sections, adult brains were fixed in 4% PFA and include

    Article Title: Learning-dependent structural plasticity of intracortical and sensory connections to functional domains of the olfactory tubercle.
    Article Snippet: For immunohistochemical analysis, dried sections were rehydrated in PBS, blocked with Tris-buffered saline with 0.2% Triton-X (TBST) containing 10% normal goat serum, and incubated with primary antibodies in blocking buffer overnight at room temperature.dried sections were rehydrated in PBS, blocked with Tris-buffered saline with 0.2% Triton-X (TBST) containing 10% normal goat serum, and incubated with primary antibodies in blocking buffer overnight at room temperature. ... The primary antibodies were mouse anti-Cre recombinase monoclonal antibody (1:200, MAB3120; Merck Millipore, Darmstadt, Germany), rabbit anti-c-fos polyclonal antibody (1:500; ab-190,289; Abcam, Cambridge, United Kingdom), mouse anti-mCherry monoclonal antibody (1:500; #632543; Takara Bio, Shiga, Japan), guinea pig antiVGLUT1 polyclonal antibody (1:500; AB5905; Merck Millipore, Burlington, MA, United States), and rabbit anti-Homer 1b/c antibody (1:200; #160023; Synaptic Systems, Goettingen, Germany).. The sections were washed with TBST and then incubated with appropriate fluorescent dye (Alexa)-conjugated secondary antibodies (1:300; Invitrogen, Waltham, MA, United States) for 1 h at room temperature.The sections were washed with TBST and then incubated with appropriate fluorescent dye (Alexa)-conjugated secondary antibodies (1:300; Invitrogen, Waltham, MA, United States) for 1 h at room temperature.

    Fluorescence:

    Article Title: A single-cell transcriptomic atlas reveals resident dendritic-like cells in the zebrafish brain parenchyma
    Article Snippet: Immunostaining was performed on 14 μm cryosections as previously described ( ).Immunostaining was performed on 14 μm cryosections as previously described ( ).. The following primary and secondary antibodies were used: chicken anti-GFP polyclonal antibody (1:500; Abcam, Cat# ab13970), rabbit anti-Lcp1 (1:1000), mouse anti-mCherry monoclonal antibody (1:500; Takara Bio Cat# 632543), Alexa Fluor 488-conjugated anti-chicken IgG antibody (1:500; Abcam Cat# ab150169), Alexa Fluor 594-conjugated anti-rabbit IgG (1:500; Abcam Cat# ab150076), Alexa Fluor 647-conjugated anti-mouse IgG (1:500; Abcam Cat# ab150107).. For vibratome sections, adult brains were fixed in 4% PFA, embedded in 7% low-melting agarose in PBS, and sectioned at 100 μm using a vibratome (Leica).For vibratome sections, adult brains were fixed in 4% PFA, embedded in 7% low-melting agarose in PBS, and sectioned at 100 μm using a vibratome (Leica).

    Article Title: Learning-dependent structural plasticity of intracortical and sensory connections to functional domains of the olfactory tubercle
    Article Snippet: with 0.2% Triton-X (TBST) containing 10% normal goat serum, and incubated with primary antibodies in blocking buffer overnight at room temperature. .. The primary antibodies were mouse anti-Cre recombinase monoclonal antibody (1:200, MAB3120; Merck Millipore, Darmstadt, Germany), rabbit anti-c-fos polyclonal antibody (1:500; ab-190,289; Abcam, Cambridge, United Kingdom), mouse anti-mCherry monoclonal antibody (1:500; #632543; Takara Bio, Shiga, Japan), guinea pig anti-VGLUT1 polyclonal antibody (1:500; AB5905; Merck Millipore, Burlington, MA, United States), and rabbit anti-Homer 1b/c antibody (1:200; #160023; Synaptic Systems, Goettingen, Germany). .. The sections were washed with TBST and then incubated with appropriate fluorescent dye (Alexa)-conjugated secondary antibodies (1:300; Invitrogen, Wal

    Article Title: A single-cell transcriptomic atlas reveals resident dendritic-like cells in the zebrafish brain parenchyma
    Article Snippet: was performed on 14 µm cryosections as described ( ). .. The following primary and secondary antibodies were used: chicken anti-GFP polyclonal antibody (1:500; Abcam, Cat# ab13970), rabbit anti-Lcp1 (1:1000), mouse anti-mCherry monoclonal antibody (1:500; Takara Bio Cat# 632543), Alexa Fluor 488-conjugated anti-chicken IgG antibody (1:500; Abcam Cat# ab150169), Alexa Fluor 594-conjugated anti-rabbit IgG (1:500; Abcam Cat# ab150076), Alexa Fluor 647-conjugated anti-mouse IgG (1:500; Abcam Cat# ab150107). .. For vibratome sections, adult brains were fixed in 4% PFA and include

    Article Title: Learning-dependent structural plasticity of intracortical and sensory connections to functional domains of the olfactory tubercle.
    Article Snippet: For immunohistochemical analysis, dried sections were rehydrated in PBS, blocked with Tris-buffered saline with 0.2% Triton-X (TBST) containing 10% normal goat serum, and incubated with primary antibodies in blocking buffer overnight at room temperature.dried sections were rehydrated in PBS, blocked with Tris-buffered saline with 0.2% Triton-X (TBST) containing 10% normal goat serum, and incubated with primary antibodies in blocking buffer overnight at room temperature. ... The primary antibodies were mouse anti-Cre recombinase monoclonal antibody (1:200, MAB3120; Merck Millipore, Darmstadt, Germany), rabbit anti-c-fos polyclonal antibody (1:500; ab-190,289; Abcam, Cambridge, United Kingdom), mouse anti-mCherry monoclonal antibody (1:500; #632543; Takara Bio, Shiga, Japan), guinea pig antiVGLUT1 polyclonal antibody (1:500; AB5905; Merck Millipore, Burlington, MA, United States), and rabbit anti-Homer 1b/c antibody (1:200; #160023; Synaptic Systems, Goettingen, Germany).. The sections were washed with TBST and then incubated with appropriate fluorescent dye (Alexa)-conjugated secondary antibodies (1:300; Invitrogen, Waltham, MA, United States) for 1 h at room temperature.The sections were washed with TBST and then incubated with appropriate fluorescent dye (Alexa)-conjugated secondary antibodies (1:300; Invitrogen, Waltham, MA, United States) for 1 h at room temperature.

    Injection:

    Article Title: A single-cell transcriptomic atlas reveals resident dendritic-like cells in the zebrafish brain parenchyma
    Article Snippet: Immunostaining was performed on 14 μm cryosections as previously described ( ).Immunostaining was performed on 14 μm cryosections as previously described ( ).. The following primary and secondary antibodies were used: chicken anti-GFP polyclonal antibody (1:500; Abcam, Cat# ab13970), rabbit anti-Lcp1 (1:1000), mouse anti-mCherry monoclonal antibody (1:500; Takara Bio Cat# 632543), Alexa Fluor 488-conjugated anti-chicken IgG antibody (1:500; Abcam Cat# ab150169), Alexa Fluor 594-conjugated anti-rabbit IgG (1:500; Abcam Cat# ab150076), Alexa Fluor 647-conjugated anti-mouse IgG (1:500; Abcam Cat# ab150107).. For vibratome sections, adult brains were fixed in 4% PFA, embedded in 7% low-melting agarose in PBS, and sectioned at 100 μm using a vibratome (Leica).For vibratome sections, adult brains were fixed in 4% PFA, embedded in 7% low-melting agarose in PBS, and sectioned at 100 μm using a vibratome (Leica).

    Article Title: Learning-dependent structural plasticity of intracortical and sensory connections to functional domains of the olfactory tubercle
    Article Snippet: with 0.2% Triton-X (TBST) containing 10% normal goat serum, and incubated with primary antibodies in blocking buffer overnight at room temperature. .. The primary antibodies were mouse anti-Cre recombinase monoclonal antibody (1:200, MAB3120; Merck Millipore, Darmstadt, Germany), rabbit anti-c-fos polyclonal antibody (1:500; ab-190,289; Abcam, Cambridge, United Kingdom), mouse anti-mCherry monoclonal antibody (1:500; #632543; Takara Bio, Shiga, Japan), guinea pig anti-VGLUT1 polyclonal antibody (1:500; AB5905; Merck Millipore, Burlington, MA, United States), and rabbit anti-Homer 1b/c antibody (1:200; #160023; Synaptic Systems, Goettingen, Germany). .. The sections were washed with TBST and then incubated with appropriate fluorescent dye (Alexa)-conjugated secondary antibodies (1:300; Invitrogen, Wal

    Article Title: A single-cell transcriptomic atlas reveals resident dendritic-like cells in the zebrafish brain parenchyma
    Article Snippet: was performed on 14 µm cryosections as described ( ). .. The following primary and secondary antibodies were used: chicken anti-GFP polyclonal antibody (1:500; Abcam, Cat# ab13970), rabbit anti-Lcp1 (1:1000), mouse anti-mCherry monoclonal antibody (1:500; Takara Bio Cat# 632543), Alexa Fluor 488-conjugated anti-chicken IgG antibody (1:500; Abcam Cat# ab150169), Alexa Fluor 594-conjugated anti-rabbit IgG (1:500; Abcam Cat# ab150076), Alexa Fluor 647-conjugated anti-mouse IgG (1:500; Abcam Cat# ab150107). .. For vibratome sections, adult brains were fixed in 4% PFA and include

    Article Title: Learning-dependent structural plasticity of intracortical and sensory connections to functional domains of the olfactory tubercle.
    Article Snippet: For immunohistochemical analysis, dried sections were rehydrated in PBS, blocked with Tris-buffered saline with 0.2% Triton-X (TBST) containing 10% normal goat serum, and incubated with primary antibodies in blocking buffer overnight at room temperature.dried sections were rehydrated in PBS, blocked with Tris-buffered saline with 0.2% Triton-X (TBST) containing 10% normal goat serum, and incubated with primary antibodies in blocking buffer overnight at room temperature. ... The primary antibodies were mouse anti-Cre recombinase monoclonal antibody (1:200, MAB3120; Merck Millipore, Darmstadt, Germany), rabbit anti-c-fos polyclonal antibody (1:500; ab-190,289; Abcam, Cambridge, United Kingdom), mouse anti-mCherry monoclonal antibody (1:500; #632543; Takara Bio, Shiga, Japan), guinea pig antiVGLUT1 polyclonal antibody (1:500; AB5905; Merck Millipore, Burlington, MA, United States), and rabbit anti-Homer 1b/c antibody (1:200; #160023; Synaptic Systems, Goettingen, Germany).. The sections were washed with TBST and then incubated with appropriate fluorescent dye (Alexa)-conjugated secondary antibodies (1:300; Invitrogen, Waltham, MA, United States) for 1 h at room temperature.The sections were washed with TBST and then incubated with appropriate fluorescent dye (Alexa)-conjugated secondary antibodies (1:300; Invitrogen, Waltham, MA, United States) for 1 h at room temperature.

    Comparison:

    Article Title: A single-cell transcriptomic atlas reveals resident dendritic-like cells in the zebrafish brain parenchyma
    Article Snippet: Immunostaining was performed on 14 μm cryosections as previously described ( ).Immunostaining was performed on 14 μm cryosections as previously described ( ).. The following primary and secondary antibodies were used: chicken anti-GFP polyclonal antibody (1:500; Abcam, Cat# ab13970), rabbit anti-Lcp1 (1:1000), mouse anti-mCherry monoclonal antibody (1:500; Takara Bio Cat# 632543), Alexa Fluor 488-conjugated anti-chicken IgG antibody (1:500; Abcam Cat# ab150169), Alexa Fluor 594-conjugated anti-rabbit IgG (1:500; Abcam Cat# ab150076), Alexa Fluor 647-conjugated anti-mouse IgG (1:500; Abcam Cat# ab150107).. For vibratome sections, adult brains were fixed in 4% PFA, embedded in 7% low-melting agarose in PBS, and sectioned at 100 μm using a vibratome (Leica).For vibratome sections, adult brains were fixed in 4% PFA, embedded in 7% low-melting agarose in PBS, and sectioned at 100 μm using a vibratome (Leica).

    Article Title: Learning-dependent structural plasticity of intracortical and sensory connections to functional domains of the olfactory tubercle
    Article Snippet: with 0.2% Triton-X (TBST) containing 10% normal goat serum, and incubated with primary antibodies in blocking buffer overnight at room temperature. .. The primary antibodies were mouse anti-Cre recombinase monoclonal antibody (1:200, MAB3120; Merck Millipore, Darmstadt, Germany), rabbit anti-c-fos polyclonal antibody (1:500; ab-190,289; Abcam, Cambridge, United Kingdom), mouse anti-mCherry monoclonal antibody (1:500; #632543; Takara Bio, Shiga, Japan), guinea pig anti-VGLUT1 polyclonal antibody (1:500; AB5905; Merck Millipore, Burlington, MA, United States), and rabbit anti-Homer 1b/c antibody (1:200; #160023; Synaptic Systems, Goettingen, Germany). .. The sections were washed with TBST and then incubated with appropriate fluorescent dye (Alexa)-conjugated secondary antibodies (1:300; Invitrogen, Wal

    Article Title: A single-cell transcriptomic atlas reveals resident dendritic-like cells in the zebrafish brain parenchyma
    Article Snippet: was performed on 14 µm cryosections as described ( ). .. The following primary and secondary antibodies were used: chicken anti-GFP polyclonal antibody (1:500; Abcam, Cat# ab13970), rabbit anti-Lcp1 (1:1000), mouse anti-mCherry monoclonal antibody (1:500; Takara Bio Cat# 632543), Alexa Fluor 488-conjugated anti-chicken IgG antibody (1:500; Abcam Cat# ab150169), Alexa Fluor 594-conjugated anti-rabbit IgG (1:500; Abcam Cat# ab150076), Alexa Fluor 647-conjugated anti-mouse IgG (1:500; Abcam Cat# ab150107). .. For vibratome sections, adult brains were fixed in 4% PFA and include

    Article Title: Learning-dependent structural plasticity of intracortical and sensory connections to functional domains of the olfactory tubercle.
    Article Snippet: For immunohistochemical analysis, dried sections were rehydrated in PBS, blocked with Tris-buffered saline with 0.2% Triton-X (TBST) containing 10% normal goat serum, and incubated with primary antibodies in blocking buffer overnight at room temperature.dried sections were rehydrated in PBS, blocked with Tris-buffered saline with 0.2% Triton-X (TBST) containing 10% normal goat serum, and incubated with primary antibodies in blocking buffer overnight at room temperature. ... The primary antibodies were mouse anti-Cre recombinase monoclonal antibody (1:200, MAB3120; Merck Millipore, Darmstadt, Germany), rabbit anti-c-fos polyclonal antibody (1:500; ab-190,289; Abcam, Cambridge, United Kingdom), mouse anti-mCherry monoclonal antibody (1:500; #632543; Takara Bio, Shiga, Japan), guinea pig antiVGLUT1 polyclonal antibody (1:500; AB5905; Merck Millipore, Burlington, MA, United States), and rabbit anti-Homer 1b/c antibody (1:200; #160023; Synaptic Systems, Goettingen, Germany).. The sections were washed with TBST and then incubated with appropriate fluorescent dye (Alexa)-conjugated secondary antibodies (1:300; Invitrogen, Waltham, MA, United States) for 1 h at room temperature.The sections were washed with TBST and then incubated with appropriate fluorescent dye (Alexa)-conjugated secondary antibodies (1:300; Invitrogen, Waltham, MA, United States) for 1 h at room temperature.



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    Image Search Results


    Reagents and tools table

    Journal: The EMBO Journal

    Article Title: Excess Met1-linked ubiquitination leads to solid aggregate formation

    doi: 10.1038/s44318-026-00909-7

    Figure Lengend Snippet: Reagents and tools table

    Article Snippet: Mouse monoclonal anti-mCherry , Clontech , 632543.

    Techniques: